![]() ![]() To display any keywords that are already in an FCS file loaded into FlowJo simply right-click on the ‘Name’ bar within the workspace and choose Edit columns from the pop-up menu that appears, as shown in Figure 3 below. Select Add Keyword from the Keyword band.Right click a population and select Add Keyword.(To access FlowJo’s shortcut key list click here.) Select a population by clicking upon it then apply shift+command+I.There are three shortcuts for entering metadata into a workspace: Notice in Figure 2 the value for the newly added keyword is only displayed on the top line corresponding to that sample.įigure 2: Keyword value only appears on the top line corresponding to a sample Thus, the keyword will only show and be editable at the sample level. Note: new keywords, like FCS keywords are associated with samples, not subsets/populations. All entries are stored with the Workspace and are available to be added to Tables and Layouts. Provide a value or other entry for the cell of the column. Click in a cell under the new keyword column in the Workspace and edit the text of the cell.Provide a name for the Keyword (this will become the name of the column displayed in the Workspace).The dialog box pictured in figure 1 will appear. Exporting statistics and table information for use in other programs like Excel, Prism, or a database If you make a steller table and need to show the world, export it in any format/file types or even loop it right back into the Layout Editor. From the Keywords band, located in the Workspace Ribbon by default, choose Add Keyword.Displaying keywords in the workspace that were either included in the FCS file at the time of collection, or added post-acquisition in FlowJo is covered on the general keywords page. For information on those keywords, go here. There are a variety of keywords that exist in FCS files by default. FlowJo makes it easy to address this and add additional keyword-value pairs to the Workspace. annotation recorded during experimental design in a spreadsheet. PreferencesĬlicking this icon opens Preferences, which can be used to fine-tune FlowJo to your specific analysis habits.There are many times when it is useful to include additional information in an analysis, e.g. The Layout Editor allows the user to create useful and attractive visual representations of data by giving the user the ability to arrange graphs, text, and other figures as desired. Layout EditorĬlicking this icon opens the Layout Editor in a new window. The Table Editor allows the user to create tables that make it easy to view, compare, and analyze different statistics of samples from the workspace. Table EditorĬlicking this icon opens the Table Editor in a new window. Groups can be created from the logical combination of their keywords or by manually dragging samples to the group. Groups are FlowJo’s mechanism to share common analysis among samples with common characteristics. How to add new samples to the workspace Create GroupĬreate a new Group definition. FCS extension, which will be added to the list of samples in the current workspace. When the Add Samples icon is clicked, a dialog box is opened in which the user can select a file with the. Pieces of text can be static, or can be dynamically generated from the properties of. Multiple fonts, styles, colors and sizes can be mixed in the same block of text. The ability to handle text is greatly improved in FlowJo X. Add SamplesĬlicking this icon allows the user to browse the local file system for samples to add to the workspace. The Text tool will adding additional text to your layout (notation, plot descriptions, etc.). The current workspace will remain open when this new workspace is created. New WorkspaceĬlicking this icon will create and open a new, empty workspace. FCS format) to add to the current workspace. This band also allows the user to create a new workspace, access preferences, add a new group to the existing workspace, or browse the file system for samples (in. From the FlowJo Navigate band, users can open FlowJo’s Table Editor and Layout Editor. ![]()
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